Small bioorthogonal Raman probes can be attached onto small drug solutes and imaged by SRS, making them ideal for drug-delivery imaging in brain.

For example, by labeling small drugs with deuterium or alkyne tags and injecting them into animal vascular system, the SRS imaging platform can visualize drug delivery across the BBB and diffusion into the brain parenchyma at subcellular resolution.​

We apply bioorthogonally labeled SRS combined with multiphoton fluorescence microscopy to investigate the structure and function of BBB in vivo by assessing the relationship between BBB permeability and neural activity-regulated signaling in vascular endothelial cells.