Multimolecular Super Resolution Metabolic Imaging in Aging and Diseases
Multimolecular Super Resolution Metabolic Imaging in Aging and Diseases
We developed a super resolution (~59 nm) multimolecular SRS metabolic imaging platform. Integrating it with Fluorescence Lifetime Imaging (FLIM), multiphoton fluorescence (MPF), and second harmonic generation (SHG), we achieved a multimodal imaging system.
We developed a super resolution (~59 nm) multimolecular SRS metabolic imaging platform. Integrating it with Fluorescence Lifetime Imaging (FLIM), multiphoton fluorescence (MPF), and second harmonic generation (SHG), we achieved a multimodal imaging system.
We discovered lipid-, protein-, carbohydrate-, and DNA/RNA-specific Raman shifts, and developed spectral unmixing methods to achieve macromolecular selectivity at subcellular resolution.
We discovered lipid-, protein-, carbohydrate-, and DNA/RNA-specific Raman shifts, and developed spectral unmixing methods to achieve macromolecular selectivity at subcellular resolution.
Schematic of workflow of 4 modality super resolution multimolecular imaging platform . Scale bar: 10 μ m
Deep Tissue Imaging Platform
Deep Tissue Imaging Platform
We are developing and applying novel deep tissue imaging platforms including:
We are developing and applying novel deep tissue imaging platforms including:
Isotope probed multiplex stimulated Raman scattering (ipm-SRS),
Isotope probed multiplex stimulated Raman scattering (ipm-SRS),
Multiphoton fluorescence (MPF),
Multiphoton fluorescence (MPF),
Enhanced stimulated Raman scattering (ESRS) microscopy.
Enhanced stimulated Raman scattering (ESRS) microscopy.
Drug Delivery
Drug Delivery
We use bioorthogonally-labeled SRS combined with multiphoton fluorescence microscopy to image drug delivery in cells, tissues, organoids, and in animals.
We use bioorthogonally-labeled SRS combined with multiphoton fluorescence microscopy to image drug delivery in cells, tissues, organoids, and in animals.
Two photon fluorescence imaging of brain microvessel ( Red is solutes injection into vessels) and astroctyes (Gcamp 6 ) in vivo in rodent.
Metabolism
Metabolism
We use label free and bioorthogonally-labeled SRS combined with multiphoton fluorescence microscopy to detect early stage in situ metabolic changes in aging, obesity, development, neurodegenerative diseases, cancer, and more.
We use label free and bioorthogonally-labeled SRS combined with multiphoton fluorescence microscopy to detect early stage in situ metabolic changes in aging, obesity, development, neurodegenerative diseases, cancer, and more.
